A variety of 25 animals, 10-week old nu/nu mice, obtained from Jackson Laboratories had been injected instantly into the kidney with luciferase-transduced hAFSC (1×106 cells/mouse diluted in 50 µl ofPBS) after glycerol damage. In vivo optical imaging was carried out with a prototype IVIS 3-dimensional bioluminescence/fluorescence optical imaging system at different time points. Absence of increases in both creatinine and BUN levels was demonstrated also in animals that received solely intra-renal injection of hAFSC with out glycerol harm, demonstrating that hAFSC alone did not alter these kidney physiological parameters over time . The presence of injected hAFSC was evaluated histologically. Frozen sections performed at 1 week after injection confirmed the presence of hAFSC, as detected by pink fluorescence of the surface marker CM-DiI .
Chicago search engine optimization Expert Search Kelowna Real Estate Listings & Homes for Sale easily. In conclusion, we have demonstrated that early direct injection of hAFSC into the kidney strongly ameliorates ATN injury, as mirrored by more speedy resolution of tubular structural injury, by tubular cell proliferation and by normalized creatinine and BUN levels. In addition, our knowledge show proof of potent immunomodulatory results of hAFSC that appear to control the local immune response in favor of a tissue cytokine and cellular milieu that promotes prevention or decision of tissue damage. Taken collectively these findings recommend that hAFSC may have therapeutic potential in ATN and, by extrapolation, perhaps in other kidney ailments. Atala, his group and we now have derived a novel stem cell population from human amniotic fluid that displays each embryonic and mesenchymal stem cell traits. An essential benefit of those cells is that they are easily retrieved through amniocentesis with no harm to the fetus.
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Carraro G, Perin L, Sedrakyan S, Giuliani S, Tiozzo C, et al. Human amniotic fluid stem cells can integrate and differentiate into epithelial lung lineages. Briefly, the cells have been incubated with a working resolution of 1 mg/ml of CM-Dil for 5 minutes at 37°C followed by an incubation of quarter-hour at 4°C and then three washes with phosphate-buffered saline [Gibco/BRL]. HAFSC, before injection, present a fibroblastoid shape as proven in Figure 1A. The cells had been then examined to verify a traditional karyotype earlier than in vivo applications, to exclude chromosomal abnormalities that would compromise their pluripotential capability . HAFSC have been analyzed for the expression of early and late kidney markers before injection.